Agar Vs Agarose. What is the difference between agar and agarose? The key difference between agar and agarose is that the agar is a gelatinous substance obtained from red algae while the agarose is a linear polymer purified from agar or red seaweeds 68 views Related Answer Visoot Phongsathorn lives in Bangkok (1963present).
PDF fileAgarose is a complex galactose polysaccharide of near neutral charge It is specially prepared and is intended mainly for use in gel electrophoresis Agar Select is recommended for molecular genetics testing Summary and Explanation Agar is a phycocolloid extracted from a group of redpurple marine algae (Class Rhodophyceae) including Gelidium.
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Agarose is obtained from agaragar by purification It makes up almost 2/3rd of the total agar content It is a linear polymer made up of the repeating monomeric unit of agarobiose Agarobiose is a disaccharide made up of Dgalactose and 36anhydroLgalactopyranose.
What is the difference between agar and agarose? Can they
Agar consists of two types of carbohydrates namely Agarose (7075%) and Agaropectin (3025%) It tends to melt at 85 °C (358 K) The composition of agar agar plays a big role in its property of easy melting and gel stability It can be ground into a fine powder So you can commonly find them in the market in the form of powders and strips.
Re: Why is agarose used in electrophoresis instead of agar
1) agaragar is preferred by vegetarians since it is seaweed based while gelatin is animal based (cow goat fish and pork) 2) another aspect is their melting point where gelatin can give the melt in your mouth effect since it melts at 35 degree celsius (important for food industry like ice cream and low fat yoghurt) while agaragar melts at 85 degree celsius.
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Agar is a jellylike material produced from red algae There are two major components of agar linear polysaccharide agarose and heterogeneous mixture of small molecules known as agaropectin This material can form the supporting structure of the cell walls of certain algae species However agar releases from the cell wall upon boiling.